Fist batch of oral reports (For Dec. 22-25, 2021)

Bio 133 Lec1 (W 4-7pm) - Oogenesis

Bio 133 Lec1 (W 4-7pm) - Oogenesis

by Ruth Maxine Flores -
Number of replies: 4

Good day, everyone!

 Here is the link for our report on the article "Molecular control of oogenesis" by Sanchez & Smitz (2012).

     https://drive.google.com/file/d/1DGoPhiZOcQai1NfLcbRTUzXtIGQ8wqyI/view?usp=sharing

If there are any questions you would like to ask us, feel free to reply in this discussion forum.

Thank you and happy holidays!

 

Members:

     Castillo, Jewell Lawrence O.

     Ching, Geanine Renee C.

     Flores, Ruth Maxine C.

In reply to Ruth Maxine Flores

Re: Bio 133 Lec1 (W 4-7pm) - Oogenesis

by Maria Clarissa Bernardo -
Hello!
I am Clarissa from Group 4.

Thank you for the very informative presentation. I was really amazed by the animations you did in some parts of your presentation, making it a bit interactive for the audience. The animations and illustrations you provided helped us visualize the processes involved in oogenesis. It was a very interesting report. I am hoping that this research could pioneer more studies that tackle medical issues in females such as PCOS and infertility.

Your group talked about cumulus-oocyte interaction in oogenesis. Our group wants to ask does cumulus-oocyte interaction affect the quality of gametes that will be produced?
In reply to Ruth Maxine Flores

Re: Bio 133 Lec1 (W 4-7pm) - Oogenesis

by Lauriz Mae Therese Avenido -
Hello Ruth, Geanine and Jewell!

I am Lauriz from Group 8.

Just as the previous comment made by Group 4, our group recognized your efforts in presenting your chosen article. We appreciate the animations and figures that made the concepts easier to understand. The reported results of this article has given further information about the present information we know about oogenesis. We also hope that this could inspire related studies about this certain topic.

As mentioned in the meiotic maturation part of your presentation, there is a question our group would like to ask. Among all of the mentioned molecules and processes involved, which plays a greater role to induce mucification or expansion of cumulus cells?

Thank you!
In reply to Lauriz Mae Therese Avenido

Re: Bio 133 Lec1 (W 4-7pm) - Oogenesis

by Geanine Renee Ching -
Hi Group 8! Thank you for that insightful question!

Among all the molecular factors that regulate the mucification/expansion of cumulus cells, GDF9 (growth differentiation factor-9), BMP15 (bone morphogenetic protein-15), and the presence of the oocyte itself are the main factors involved in the process. GDF9 and BMP15 are known as cumulus expansion enabling factors (CEEFs). They stimulate the expression of the other transcripts mentioned in the video, including Has2, Tnfaip, Ptx3 and Ptgs2. Therefore, without the two factors, cumulus expansion will not be initiated at all. This may eventually lead to female infertility, as evidenced by some studies (Elvin et al., 1999; Yoshino et al., 2006).

On the other hand, presence of the oocyte itself is also necessary for cumulus cell differentiation and expansion. In oocytectomy procedures (OOX), where fully-grown oocytes are removed from the oocyte-cumulus complex, cumulus cells were observed to dedifferentiate. This entirely prevents the mucification and further development of cumulus cells. Despite all this, each molecular factor that regulates cumulus cell mucification is important for normal oogenesis to occur. Mutations or deficiencies in any of these factors will definitely have deleterious effects on the developing oocyte.
 
References:

Elvin, J. A., Clark, A. T., Wang, P., Wolfman, N. M., & Matzuk, M. M. (1999). Paracrine actions of growth differentiation factor-9 in the mammalian ovary. Molecular Endocrinology, 13(6), 1035–1048. https://doi.org/10.1210/mend.13.6.0310 

Yoshino, O., McMahon, H. E., Sharma, S., & Shimasaki, S. (2006). A unique preovulatory expression pattern plays a key role in the physiological functions of BMP-15 in the mouse. Proceedings of the National Academy of Sciences, 103(28), 10678–10683. https://doi.org/10.1073/pnas.0600507103 

In reply to Ruth Maxine Flores

Re: Bio 133 Lec1 (W 4-7pm) - Oogenesis

by Lydia LEONARDO -
Thank you for a very clear presentation of the intricate interplay of factors during oogenesis. My questions are:
1. You mentioned the ability of the oocyte support embryo development as influenced by oocyte-cumulus contact. Can you please explain this a little more?
2. Are there aspects in this interplay of factors that have been exploited for contraception?
Thanks.