Methods in Studying Proteins
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Isoelectric focusing
Another form of electrophoresis is isoelectric focusing, where the samples are run through a gel matrix that has different pH.
Building on isoelectric focusing is 2-dimensional PAGE (2D-PAGE), a method which involves both isoelectric focusing and SDS-PAGE (starts at 7:00 in the video).
*Note: anode = (+) electrode; cathode = (-) electrode
What do you think is this separation based on?