Methods in Studying Proteins
View
Go through the activity to the end
You have earned 12 point(s) out of 13 point(s) thus far.
Determining Protein Sequence
The primary structure of protein or peptide can be determined by several ways. One of these is by taking advantage of the selective cleavage of the enzymes (like trypsin and chymotrypsin) or chemicals (like cyanogen bromide).
The diagram below summarizes the cleavage properties of these enzymes/chemicals.
As an example of using these components, shown below is the cleavage of peptide:
From knowing these cleavage properties, we can then determine the sequence of the amino acids in a protein.
Here is a list of more enzymes and chemicals that can be used to determine protein sequence through their cleavage properties. Please go through the list and familiarize yourself with these reagents.