Activity 6: Guide
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Guide Questions and References
Guide Questions
What is the basis for fluorescence quenching?
How are protein interactions investigated using fluorescence?
References
Chemistry Unit (2020). Laboratory Manual for Biochemistry Majors.
Chateller, D. S., & White, H. B. (1988). What color is egg white? A biochemical demonstration of the formation of a vitamin protein complex using fluorescence quencing. Journal of Chemical Education, 65 (9), 814–815.
Henderleiter, J.A., Hyslop, R. M. (1996). The Analysis of Riboflavin in Urine Using Fluorescence. Journal of Chemical Education, 73 (6)(563-564).
Tillotson, J. A., & Bashor, M. M. (1980). Fluorometric apoprotein titration of urinary riboflavin. Analytical Biochemistry, 107, 214–219.