Activity 6: Guide
Fluorescence Data of 3 Vitamin Samples
Three (3) vitamin samples were analyzed: Ironvit, Revicon, and Enervon. Fluorescence data for each are found below. This data came from RDelaCruz, KLim, and APadaong (BS Biochemistry, Batch 2017).
Note: You will be writing a formal report base on the protocol and data found here.
Appendix A. Relative Fluorescence of Ironvit upon addition of different volumes of egg white. EX=280nm, EM=535nm.
|
Tube # |
Vol. of Egg white (μL) |
Fluorescence |
|
1 |
0 |
3258 |
|
2 |
300 |
647.449 |
|
3 |
600 |
387.369 |
|
4 |
900 |
357.764 |
|
5 |
1200 |
317.678 |
|
6 |
1500 |
309.325 |
|
7 |
1800 |
296.406 |
|
8 |
2100 |
172.746 |
|
9 |
2400 |
148.219 |
|
10 |
2700 |
120.143 |
Appendix B. Relative Fluorescence of Revicon upon addition of different volumes of egg white. EX=280nm, EM= 535nm.
|
Tube # |
Vol. of Egg white (μL) |
Fluorescence |
|
1 |
0 |
21844.089 |
|
2 |
300 |
4076.37 |
|
3 |
600 |
1543.834 |
|
4 |
900 |
548.022 |
|
5 |
1200 |
399.905 |
|
6 |
1500 |
290.785 |
|
7 |
1800 |
124.366 |
|
8 |
2100 |
100.944 |
|
9 |
2400 |
77.762 |
|
10 |
2700 |
32.648 |
Appendix C. Relative Fluorescence of Enervon upon addition of different volumes of egg white. EX=280nm, EM = 535nm.
|
Tube # |
Vol. of Egg white (μL) |
Fluorescence |
|
1 |
0 |
2592.290 |
|
2 |
300 |
2100.424 |
|
3 |
600 |
2084.261 |
|
4 |
900 |
2077.237 |
|
5 |
1200 |
2026.389 |
|
6 |
1500 |
1951.600 |
|
7 |
1800 |
1673.117 |
|
8 |
2100 |
1388.528 |
|
9 |
2400 |
1390.824 |
|
10 |
2700 |
1142.439 |