Activity 3: Guide
View
Go through the activity to the end
SDS-PAGE video
Watch this video and take note of the steps and reagents involved in the process. You can also download the SDS-PAGE protocol we do in the Chem lab to guide you through the process in the video.
Notes:
Lower stock = 4X running gel buffer
Upper stock = 4x stacking gel buffer
The running gel can also be called the resolving gel or the separating gel.
In the lab, we don't store our gels for long as shown in the video, we usually use them immediately, or store them for 2 days max.