Course Guide
This contains the topics and goals of the course.
3. Weekly Schedule
This shows the weeks where specific modules will be taken up.
|
Week No. |
Topic |
Topic Outcome |
Time Frame (hr) |
Learning Activities |
Learning Resources |
|
1 |
ORIENTATION
|
|
1.5 |
Synchronous through Zoom or other online meeting platform |
- Course Guide |
|
2 |
Protein Isolation and Quantitation |
- To explain the steps and principles in protein isolation - To evaluate protein yield |
3.0 |
Watch Video/ Perform Virtual Simulation
Submit data sheet/probset/ formal report |
- Online videos/ references - Module - Activity guide - Lab protocol |
|
3 |
Spectrophotometric Determination of Iron(III)-Glycine Formation Constant |
- To explain the concepts in metal-ligand binding and the principles behind metal-ligand coordination chemistry - To compute for metal-ligand formation constant from spectrophotometric data |
3.0 |
Watch Video/ Perform Virtual Simulation
Submit data sheet/probset/ formal report |
- Online videos/ references - Module - Activity guide - Lab protocol |
|
4 |
Protein Visualization using SDS-PAGE and other variations |
- To explain the principles behind discontinuous SDS-PAGE - To analyze and interpret protein profiles |
3.0 |
Watch Video/ Perform Virtual Simulation
Submit data sheet/probset/ formal report |
- Online videos/ references - Module - Activity guide - Lab protocol |
|
5 |
Western blotting and ELISA |
- To explain the rationale behind Western Blotting and ELISA - To interpret blot profiles and ELISA results - To expand on the applications of western blotting and ELISA |
3.0 |
Watch Video/ Perform Virtual Simulation
Submit data sheet/probset/ formal report |
- Online videos/ references - Module - Activity guide - Lab protocol |
|
6 |
Protein Crystallization |
- To explain the rationale behind hanging drop vapor method in crystallization - To compare and differentiate different precipitants in their ability to crystallize proteins - To identify the best conditions for protein crystallization |
3.0 |
Watch Video/ Perform Virtual Simulation
Submit data sheet/probset/ formal report |
- Online videos/ references - Module - Activity guide - Lab protocol |
|
7 |
Relative Quantification of Riboflavin Content of Commercially Available Food Supplements using Fluorometric Quenching Titration |
- To explain the concept behind fluorometric quenching - To quantify (relatively or absolutely) the amount of riboflavin in different samples - To explain how protein interactions can be observed through fluorescence |
3.0 |
Watch Video/ Perform Virtual Simulation
Submit data sheet/probset/ formal report |
- Online videos/ references - Module - Activity guide - Lab protocol |
|
8 |
ORAL REPORT |
|
3.0 |
|
|
|
9 |
Characterizing Protein structure through Mass Spectrometry data |
- To determine protein size through mass spectrometry (ES +) data - To determine peptide sequence through MS data |
3.0 |
Watch Video/ Perform Virtual Simulation
Submit data sheet/probset/ formal report |
- Online videos/ references - Module - Activity guide |
|
10 |
Predicting protein structure using Online Bioinformatics tools |
- To determine protein structure based on metagenomics data |
3.0 |
Watch Video/ Perform Virtual Simulation
Submit data sheet/probset/ formal report |
- Online videos/ references - Module - Activity guide - Lab protocol |
|
11 |
JOURNAL REPORT |
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|
12-13 |
MINI-PROPOSAL SUBMISSION/ COMPLETION OF REQUIREMENTS |
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